The Department has added many new and unique lab tests recently. The following are the new additions:

• MTB/NTM Real Time PCR
• Mycoplasma pneumoniae/Chlamydia pneumoniae Real Time PCR
• HSV1/2 Real Time PCR
• Chlamydia T./Neisseria G./Mycoplasma G./ Trichomonas V.
• Real Time PCR
• HPV Real Time PCR (High Risk Group)
• HPV Real Time PCR (low risk group)
• HPV 16/18 typing
• HBV Real Time PCR, Quantitative
• HCV Real Time RT-PCR, Qualitative
• HCV Real Time RT-PCR, Quantitative
• HCV Genotyping
• HIV-1 Real Time RT-PCR, Quantitative
• CMV Real Time PCR
• EBV Real Time PCR
• CMV/EBV Real Time PCR
• CMV/EBV/HHV-6 Real Time PCR
• CMV Real Time PCR Quantitative
• EBV Real Time PCR Quantitative

HLA related tests

• HLA B27 DNA Detection

Leukemia Associated Translocation PCR

• Real Time PCR for 7 translocation – t (9,22) minor, major, and micro; t (1,19); t(12,21); inv16; t(15,17) S, V and L; t(8,21) and t(4,11)
• Real Time PCR for (BCR-ABL1) (Major, minor, micro), t (8;21) (RUNX1-RUNX1T1), inv16(CBFB-MYH11)
• Real Time PCR for BCR-ABL1 (3 variants), t (4;11) (MLL-AFF1), , t(1;19)(TCF3-PBX1), t(12;21)(ETV6-RUNX1)
• Real Time PCR for PML-RARA (3 variants)

JAK-2 mutation PCR in Myeloproliferative Disorders

Flow Cytometric Immunophenotyping

1. Immunophenotyping – Acute Leukemia full Panel(CD45, CD34, HLA-DR, CD13, CD33, CD117, MPO, CD10, CD19, CD79a, CD3, CD4, CD5, CD7, CD8, cyCD3, CD14, CD64)
2. Immunophenotyping – Acute Leukemia basic Panel(CD45, CD34, HLA-DR, CD13, CD33, CD117, CD10, CD19, CD20, CD3, CD5, CD7)
3. Immunophenotyping – Multiple Myeloma(CD45, CD19, CD117, CD38, CD56, CD138, Anti-Kappa, anti-Lambda)
4. Immunophenotyping – HIV(CD45, CD3, CD4, CD8)

Tuberculosis

Tuberculosis (TB) is one of the world’s most common severe infectious diseases. Approximately 2 million deaths per year are TB-related. A single patient with an active TB may infect 10 to 15 other people each year. In the fight to stop the spread of TB the most important milestone is early diagnosis. However, diagnosis of TB is a challenge. Evercare Molecular Lab is pioneer in detecting TB from any kind of specimen. Moreover, we can simultaneously detect non-tuberculous mycobacterium (NTM). We already have detected TB/NTM by real time PCR from sputum, BAL, any aspirate, body fluids, CSF, urine, tissue, tissue block (paraffin block), pus and are accepting these specimens for test.

Atypical Pneumonia

Only we are doing multiplex PCR for simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae which causes atypical pneumonia.

Virus Detection and Quantification by Real Time PCR

Precise and accurate detection of viral load and its genotypes ensures confidence in results. Our virological test systems include hepatitis B, C for hepatitis, CMV/EBV/HHV-6, and HIV for immunocompromised patient, HPV for cervical cancer and HSV 1/2 for CNS, eye and genital infections by Real Time PCR enable you a reliable detection of virus which is available only in Evercare Hospital Dhaka.

• CMV Real Time PCR
• EBV Real Time PCR
• CMV/EBV Real Time PCR
• CMV/EBV/HHV-6 Real Time PCR
• CMV Real Time PCR Quantitative
• EBV Real Time PCR Quantitative
• HSV1/2 Real Time PCR
• HPV Real Time PCR (High Risk Group)
• HPV Real Time PCR (low risk group)
• HPV 16/18 typing
• HBV Real Time PCR, Quantitative
• HCV Real Time RT-PCR, Qualitative
• HCV Real Time RT-PCR, Quantitative
• HCV Genotyping
• HIV-1 Real Time RT-PCR, Quantitative

Detection of Sexually Transmitted Infections

Neisseria gonorrhoeae, Chlamydia trachomatis, mycoplasma genitalium, Trichomonas vaginalis are the most common pathogens that cause sexually transmitted bacterial infection (STI). The organism causes genitourinary infections in women and men and may be associated with dysuria and vaginal, urethral, or rectal discharge and many times do not produce any symptoms initially.

In women, complications include pelvic inflammatory disease, salpingitis, and infertility. Approximately 25% to 30% of women who develop acute salpingitis become infertile. Complications among men are rare but include epididymitis and sterility. PCR is the most sensitive and specific method for the detection of these pathogens and is now recommended for diagnosis.

Cervical Cancer Screening

Cervical cancer is the third most prevalent cancer in women around the world, and the fourth highest cause of women’s cancer-related deaths. The link between cervical cancer and human papillomavirus (HPV) has become clear over the past few decades—today we know that persistent infection with specific types of HPV account for nearly all cases of cervical cancer.

Because cervical cancer rarely causes overt symptoms in its early stages—when treatment is most effective—screening for HPV infections at the greatest risk of progressing to cervical pre-cancer and cancer is imperative. 14 high-risk HPV types are detected in nearly 100% of cervical cancers. Of these, two types are associated with 70% of cervical cancers. Type 16 causes approximately 55% to 60% of cervical cancer cases, and type 18 accounts for 10% to 15%. Real time PCR is the most sensitive and specific method for the detection of HPV.

• HPV Real Time PCR (High Risk Group)
• HPV 16/18 typing
• HPV 6/11 Real Time PCR for wart

Lab Medicine Department of Evercare Hospital Dhaka is committed to assuring quality and maintaining JCI (Joint Commission International) standards. The following are our quality assurance programs:

Pre-Analytical Quality Control

• Patient identification by UHID and Patient name from wrist band/ registration card
• Universal precaution
• Collection of samples in appropriate vacutainer/ appropriate container
• Bar code labeling and transport to the lab

Analytical Quality Control

• Man behind the machine and appropriate technology
• Internal Quality Control: Two levels of quality control materials run every day in the hospital
• External Quality Control Program with BIO-RAD, USA every month
• Proficiency Testing with Bangabandhu Sheikh Mujib Medical University & AFIP

Post-Analytical Quality Control

• Finalization of test result by Registrar/Consultant after viewing the history in HIS
• Effective communication: Critical Call Back
• Fastest Turn Around Time
  • Emergency: 45 minutes to 1 hour
  • IPD: 1 hour to 2 hours
• Clinico-pathological conversation/meeting with clinical counterpart.

New Quality Control Parameters

The hospital has introduced a range of newer parameters for quality control which are not done anywhere else in Bangladesh. These include:

• New studies
  • Cystatin C
  • Procalcitonin
  • Lipoprotein A
  • NT-pro BNP
  • Homocysteine
  • Protein C, Protein S
  • Platelet Aggregation Studies
  • HLA-B27
  • Anti-CCP
  • TB IgM, IgG IgA
  • BV
  • ENA Profile
  • C1 Esterase Inhibitor
• 24-hour Frozen Section 
• Review of Histopathology slides and blocks from outside sources for second opinion.
• Introduction of External Quality Control/ Profanely Testwithin the country (with Bangabandhu Sheikh Mujib Medical University and AFIP) and outside the country (with BIO-RAD Laboratory, California, USA). Histopathology Quality Control is done by inter-institutional review for some selected cases.

We have developed Critical Call Back protocol, which is regularly maintained in the hospital. We are proud to be the pioneer in Bangladesh to introduce the system of Critical Call Back in Lab Medicine Department of Evercare Hospital Dhaka, which was highly appreciated by the survey team of Joint Commission on Accreditation of Healthcare Organizations (JCAHO) during our accreditation process.